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Atlas of Comparative Diagnostic and Experimental Hematology, 2nd Edition
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Atlas of Comparative Diagnostic and Experimental Hematology, 2nd Edition


ISBN: 9781405171076
Hardcover
152 pages
July 2011, Wiley-Blackwell


Description
A vital resource on blood and bone marrow cell morphology in laboratory animal medicine. This fully revised new edition is an essential reference for clinical pathologists in diagnostic laboratories, and medical or veterinary research. The atlas contains over 400 color images of cells from the peripheral blood and bone marrow from a variety of animals encountered in laboratory animal medicine, in health and disease.

Key features:

  • New chapter on flow cytometry and its application in terms of routine analyses as a means of identifying abnormalities in cell marker expression, which is of particular relevance for pre-clinical safety assessment
  • Covers the most recent developments in laboratory animal hematology, including parameters measured by the latest generation of analyzers
  • Coverage of a wide range of laboratory animal species, as well as those used in clinical veterinary trials
  • Photomicrographs present normal and abnormal blood cells from a variety of hematological conditions along with descriptive text



    Table of Contents
    Foreword vii

    Introduction ix

    Dedication xi

    Acknowledgments xiii

    1 Rodents 1

    Mouse 1

    Introduction 1

    Blood picture 1

    Typical ranges (Siemens Advia 120) 2

    Hamster 15

    Introduction 15

    Blood picture 15

    Guinea Pig 17

    Introduction 17

    Blood picture 17

    Rat – Sprague Dawley (SD)/Han Wistar (HW) 18

    Introduction 18

    Blood picture 18

    Typical ranges (Siemens Advia 120) 19

    References 36

    2 Rabbit 37

    Introduction 37

    Blood picture 37

    References 39

    3 Cat 41

    Introduction 41

    Blood picture 41

    References 44

    4 Dog 45

    Introduction 45

    Blood picture 45

    Typical ranges (Siemens Advia 120) 46

    References 64

    5 Monkey 65

    Introduction 65

    Blood picture 65

    Marmoset 66

    Cynomolgus 71

    Introduction 71

    Blood picture 71

    Typical ranges (Siemens Advia 120) 72

    Baboon 84

    Rhesus Monkey 86

    References 87

    6 Other species 89

    Pig 89

    Introduction 89

    Blood picture 89

    Horse 91

    Introduction 91

    Blood picture 91

    Goat and Sheep 94

    Assorted Other Species 96

    References 103

    7 Blood collection procedures 105

    Common bleeding sites 105

    Anticoagulants 105

    Staining 105

    8 Artifacts 107

    Collection artifacts 107

    Sample transport and storage 109

    Anticoagulant 110

    Storage artifacts 111

    Blood smear preparation 113

    Stain precipitate 113

    References 114

    9 Bone marrow 115

    Introduction 115

    Review of marrow sampling techniques 115

    Recommended methods for marrow sampling 116

    Preparation and staining of smears 118

    Evaluation 119

    Interpretation 119

    References 120

    10 Comparative applications in flow cytometry 121

    Application of fl ow cytometry in comparative hematology 122

    References 132

    Index 135


    Author Information
    Clifford Smith is Chief Laboratory Scientist in the Haematology Department, and Head of Logistics, Clinical Pathology Services, at Covance Laboratories in Harrogate, UK.

    Alfred Jarecki is Principal Research Scientist with Sanofi-Aventis at the Alnwick Research Centre, UK.

 
 
 
 
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